Cuckoo, or Rhododendron species, is a unique plant known for its small leaves and flowering period typically between March and April. Based on this characteristic, it's likely that the larger variety could be Dongyang, while Xiqiao remains a possibility. However, due to the extremely fine seeds of azaleas, growing them from seed can be quite challenging, especially in home environments. The process requires strict conditions such as soil sterilization, maintaining high humidity, and controlled temperature—factors that are often difficult to achieve without professional equipment. Therefore, propagation through cuttings is generally recommended. If suitable rootstocks are available, grafting can also be an effective method.
From May to June, select short, semi-woody branches from the current year. Cut these from the base, leaving 3 to 5 sets of leaves at the top. If the branches are too long, you can trim the tips. For cutting media, options include orchid mud, alpine humus, vermiculite, perlite, or yellow heart soil. The medium should be about 15 to 20 cm thick, with a 7 to 8 cm drainage layer at the bottom. Once the medium is moist, insert the cuttings up to half their length. For small-scale propagation at home, large flower pots can be used to hold the cuttings. After planting, cover the container with plastic film to maintain moisture, keep the temperature between 25°C and 30°C, and place it in a shaded area or under a shade cloth to avoid direct sunlight. Keep the substrate consistently moist throughout the rooting process.
Rooting usually takes around one month for East Wolfberry varieties, while Xiqiao may take 60 to 70 days. After about 9 months, you can gradually reduce the shading time and apply a diluted urea solution (around 0.2%) as a top dressing. By late October, the plants can be transferred into pots. In commercial settings, using an intermittent mist system with vermiculite as the medium, cuttings can survive within 20 days, achieving a survival rate of up to 95%.
Additionally, it's worth noting that in regions like Northeast China, Inner Mongolia, Russia, and North Korea, there exists a wild variety called *Rhododendron parrifolum*, which is an evergreen shrub with a rose-colored corolla. This differs from the colorful varieties commonly described, so it can be ruled out as a match for the plants being discussed.
Nucleic Acid (DNA/RNA) Extraction Kit
1. Introduction
The total viral nucleic acid extraction kit is suitable for extracting total viral nucleic acid from serum, plasma, tissue homogenate and other samples. The kit is based on silica column purification technology, which eliminates the need for toxic phenol-chloroform extraction and time-consuming alcohol precipitation. This product has successfully extracted nucleic acids from hepatitis B A/C, hepatitis C, and norovirus standard. The obtained DNA/RNA can be directly used in a series of downstream experiments such as PCR, RT-PCR, and LAMP.
Notice:
1. The carrier RNA solid must be dissolved in Nuclease Free Water to 1µg/µl before use, and vortex to dissolve. Store in aliquots at -70°C. If you need to store it at -20℃ for a long time, please repackage it according to the number of times of use.
2. Dissolve Proteinase K (20mg/ml): Add Proteinase Dissolve Buffer to dissolve Proteinase K to a final concentration of 20mg/ml. Proteinase K dry powder can be stored at 2-8°C for one year, but dissolved Proteinase K must be stored in aliquots at -20°C. Repeated freezing and thawing of Proteinase K can affect its activity.
3. Buffer VHB must be diluted with 14 ml absolute ethanol before use and stored at room temperature.
4. Buffer RW2 must be diluted with 80 ml of absolute ethanol before use and stored at room temperature.
3. Shelf life
Except for Proteinase K and Carrier RNA, other components of this product can be stored at room temperature (15-25°C) for 12 months, and should be stored at 2-8°C for long-term storage. Proteinase K and Carrier RNA dry powder are transported at room temperature. Please store at -20°C after receiving the test product, and store at -20°C after dissolving.
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