Rhododendron, commonly known as Cuckoo, is a unique plant species characterized by its small leaves and a flowering period that typically occurs from March to April. Based on this information, it's reasonable to assume that the larger variety might be Dongyang, while the possibility of Xiqiao cannot be ruled out entirely. The seeds of azaleas are extremely tiny, making them very sensitive during the sowing process. Factors such as soil sterilization, maintaining high humidity, and controlled environmental conditions are crucial, which are often difficult to achieve in home settings. Therefore, propagation through cuttings is highly recommended. If suitable rootstocks are available, grafting can also be an effective method.
From May to June, you should select semi-woody branches from the current year that are relatively short. Cut them from the base, leaving 3 to 5 sets of leaves at the top. If the branches are too long, trim the tips to make them more manageable. For the cutting medium, you can use orchid mix, alpine humus, vermiculite, perlite, or yellow heart soil. The depth of the medium should be around 15 to 20 cm, with a drainage layer of about 7 to 8 cm at the bottom. After moistening the medium, insert the cuttings up to about half their length. For small-scale propagation at home, large flower pots can also be used to hold the cuttings. Once planted, cover the container with plastic film to maintain moisture, keep the temperature between 25°C and 30°C, and place it in a shaded area or under a shade net to avoid direct sunlight. The substrate should remain consistently moist throughout the rooting process.
Rooting usually takes about one month for the East Wolfberry variety, while Xiqiao may take 60 to 70 days. After approximately nine months, you can gradually reduce the shading time and apply a diluted urea solution (about 0.2%) as a foliar feed. By late October, the plants can be transplanted into pots. In commercial settings, using an intermittent misting system with vermiculite as the cutting medium can result in a survival rate of up to 95% within 20 days.
It’s also worth noting that in regions such as Northeast China, Inner Mongolia, Russia, and North Korea, there exists a wild variety of Rhododendron parrifolum, which is an evergreen shrub with rose-colored flowers. This differs from the colorful varieties you've described, so it can be excluded from consideration.
DNA/RNA Purification Kit
The RNA purification kit is used to purify and recover RNA molecules transcribed in vitro and total RNA extracted from various materials, which can effectively remove contaminating impurities in RNA samples. The recovery rate of this product can reach 80%, and the OD260/OD280 ratio of the obtained RNA is generally about 2.0, which can be directly used in subsequent sensitive experiments (such as microarray analysis, fluorescence RT-PCR, etc.). With the deepening of transcriptomics, the complexity of RNA types, expression regulation and functions is far beyond our imagination.
Removing ribosomal RNAs that account for more than 80% helps to focus sequencing on less abundant but informative RNAs. At present, the removal of rRNA is mainly through the combined use of probe and RNase H. The processed RNA will be mixed with many digestion products, enzymes and ions, which is not conducive to the subsequent construction of RNA library. Take the Columnar RNA Purification Kit as an example. Trizol is a ready-to-use reagent that can be used to purify total RNA from tissues and cells. This is a single-phase solution of phenol and guanidine isothiocyanate that facilitates lysis of tissues and cells, inhibiting RNases to maintain RNA integrity.
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